The dedicated leica application suite las x software platform facilitates the setup of even complex experiments. View and download leica dmi8 instructions for use manual online. While telescopes were the original focus of the business, microscopes soon became the primary product. Leica microsystems fluorescence stereo microscopes use the triplebeam technology, a separate third beam path for fluorescence illumination of the sample without a dichroic mirror. User manual leica dmi6000 microscope university of arizona. Zeiss axio observer z1 manual single or tile imaging with zen.
Leica sp5 inverted confocal manual duke light microscopy. View and download leica cme instruction manual online. Intended purpose of microscopes the leica dm6000 b microscope, to which this user manual belongs, is designed for biological routine and research applications. Leica dm500 leica fluorescence microscope microscope. Leica biosystems provides you with wide range of fluorescence imaging capabilities starting with fluorescence microscopes. Binocular, fluorescencecapable educational microscope for life science courses leica dm500. Superior optics, ergonomics, automation and image quality make leica biosystems your imaging solution provider of choice. This includes the examination of samples taken from the human body with a view to providing information on physiological or pathological states or congenital abnormalities, or to determining the. Fluorescence microscopes leica dmil inverted fluorescence. Instruction manual leica dmre fluorescence microscope. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. This text is a guide to operating the leica fluorescence microscope dm6000.
Roger robbins 5172006 table of contents leica inm100 optical microscope operation manual purpose introduction microscope description general microscope operation startup stage positioning lighting read more. Find online auctions and classified ads for leica fluorescence microscopes on the labx. Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed. Jul 11, 2016 leica dm il fluorescence microscope available from the lab world group. Leica inm100 optical microscope operation manual cleanroom. Leica stereo fluorescence systems the school of molecular and. M stereomicroscopes 73 pages microscope leica dmi8 instructions for use manual 98 pages microscope leica dmire2 manual. If the next user is coming within approximately 1hr and will be using fluorescence leave the microscope, lamp, camera and computer on.
Las x software leica biosystems application suite advanced fluorescence las x is the easy to use software platform for advanced fluorescence imaging. The leica dm il led features a comprehensive set of contrast methods to monitor your specimen the way you need. Fit microscope carrier of leica mz10 f to focusing drive in accordance with user manual m21050en. Performance data of the incident light fluorescence illumination n.
This comes with your choice of hi plan or n plan brightfield objectives. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence. Page 17 assembly light sources for incident light fluorescence before assembling to the microscope, check if the leica dm ls microscope can be equipped the lamps have already been inserted fig. The images were captured with a leica m165 fc stereo microscope where a leica 2x plan apo objective with right image and without left image correction collar was used. For incident light fluorescence, mercury and xenon. The leica mz fliii fluorescence stereomicroscope offers not only a 3d image but also, compared with a classical microscope at any given magnification, a larger panoramic field of view, more intense fluorescence. The leica dmirb respectively the leica dm ire2 is the logical further development of the successful inverted research microscope from leica. Capture faintest signal and highly dynamic processes with newest scmos sensor technology. A leica dm ls microscope instruction manual is included for reference only. It provides users with a powerful imaging tool in an accessible workflow based design.
The leica dm l microscope is fitted for incident light bf, df, pol and icr with a 12 v 100 w halo gen lamp. View and download leica dm irb instructions manual online. Widely recognized for optical precision and innovative technology, leica microsystems is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning and superresolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy. Leica am total internal reflection fluorescence manual duke.
A fluorescence microscope uses a higher intensity light to illuminate the samples. Leica dm il the department of physiology and biophysics. Leica high quality opticshave set global standards in optics engineering. An automated fluorescence microscope that offers similar functionality to a confocal, live cell incubator, and slide scanner.
Leica microsystems has developed the m205 fa and m205 fca fluorescence stereo microscopes to enable you to detect transgenic expression like gfp and mcherry in early stages, allowing you to select the right sample to successfully base your studies on. The intensity is individually adjusted and stored for each objective and contrast method. Leica is a leader in the field of microscopy and has been providing innovative solutions to the. A more detailed and thorough account of these procedures can be found in the leica dm6000 b operating manual. Fluorescence video imaging of a zebrafish larva expressing green fluorescent protein gfp immersed in an aqueous solution. With the help of superresolution fluorescence microscopy, it is even possible to image subresolution structures. It applies to the microscope leica dmlb mainly for biological and medical applications and, together with a supplementary manual, to the microscopes dmlp polarized light and dmlm material examinations.
Leica microsystems has developed the m205 fa and m205 fca fluorescence stereo microscopes to enable you to detect transgenic expression like gfp and mcherry in early stages, allowing you to. This device and the filter cubes are identical to the corresponding components of leica microscopes. This user manual describes the special functions of the individual modules of the leica mz10 f fluorescence stereomicroscope and the leica fluorescence module for the leica m stereomicroscopy series and contains important instructions for their operational safety, maintenance, and. Leica fluorescence microscope the leica mz10 f modular stereomicroscope with 8x80x magnification and 375lpmm high resolution provides triple beam technology, which designates a separate beam path specifically for fluorescent illumination for high contrast and detailed fluorescent imaging. Fluorescence microscopes leica dmlb fluorescence phase. Las x software leica biosystems application suite advanced fluorescence las x is the easy to use software platform for advanced fluorescence. Microscope central is proud to offer an extensive line of leica microscopes, cameras, and accessories. Olympus ix70 inverted fluorescent microscope manuals. Permitted uses leica stereofluorescence systems are equipped with a special fluorescence illuminator.
We have this preconfigured with your choice of a binocular, ergo binocular, or trinocular viewing head. Leica dmlb fluorescence microscope microscope central. Leica dmre microscope photometrics coolsnap ccd fx camera imaging options. Leica dfc9000 scmos fluorescence microscope camera enables imaging cells under nearnative conditions. Fluorescence microscope binocular microscopes for sale ebay. Microscopes and imaging systems leica microsystems. Check log sheet to see the notes of previous users. Lighting a key feature for any comparison microscope, one. Permitted uses leica stereo fluorescence systems are equipped with a special fluorescence. Leica microsystems offers a wide range of microscope fluorescence filters that can be used with existing fluorescence filters to create the best spectral properties of a specimen. Cell imaging unit uic 12 pages microscope leica m420 instruction manual. This user manual describes the special functions of the individual modules of the leica mz10 f fluorescence stereomicroscope and the leica fluorescence module for the leica.
O the leica accessories described in this user manual. The led fluorescence illumination eliminates the warm upcool down period, bulb changes, and is preset with no centering necessary. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. The leica dmlb fluorescence microscope can be fully customized to meet the needs of your laboratory. The leica dm500 microscope with plug and play capability is the ideal tool to make teaching entrylevel college and university life science courses easy and fun for the instructor and the student. O other accessories, provided that these have been expressly approved by leica as being technically safe in this context. Make sure the bottom of the coverslip and the objective are clean, any dirt can make tirf illumination uneven. The leica mz fliii fluorescence stereomicroscope offers not only a 3d image but also, compared with a classical microscope at any given magnification, a larger panoramic field of view, more intense fluorescence, and longer working distances. Leica dfc9000 scmos fluorescence microscope camera. Minimanual how to use the leica dm2500 microscope transmission light microscopy bf, ph, df x put the sample on the stage holder 12 and move the sample with the xy stage device 8 to the center of the field of view x open the transmission light switch 6 and adjust the intensity of the lamp 3.
Leica am total internal reflection fluorescence manual. User manual the present user manual describes only the functions of the fluorescence illuminator, the use of the filter sets, and the fitting of the fluorescence module. For everything you need to know about the correct use of fluorescencerelated parts, refer to. Whether you need an entrylevel solution or an advanced analysis and documentation package, leica microsystems provides ergonomicallydesigned manual stereo microscopes for routine tasks. Full control over your samples with manual ergonomic clinical microscope systems.
All led systems are 5 watt with 50,000 hour lifespan. This user manual describes the special func tions of the individual modules of the leica. The leitz microscope traces its lineage to a company founded in 1849 in wetzlar, germany, the optical institute. The leica dm6000 b microscope, to which this user manual belongs, is designed for biological routine and research applications. Leica dm2500 upright fluorescent microscope manuals. Leica dm500 user manual safety regulations 14 accessories, maintenance and repair accessories only the following accessories may be used with the leica dm500 microscope.
This user manual describes the special functions of the individual modules of the leica mz10 f fluorescence stereomicroscope and the leica fluorescence module for the leica m stereomicroscopy series and contains important instructions for their operational safety. The leica dmi6000b microscope is part of the imaging cores life sciences north. The stereo fluorescence modul can be combined with the leica stereomicroscope models ms5, mz6, mz7. These can be found and purchased separately by others. User manual leica dmi6000 microscope the microscopy alliance. Leica biosystems provides you with wide range of fluorescence imaging capabilities starting with fluorescence microscopes through dedicated integrated fish capture stations, to fish scanning solutions. High intensity fluorescence with zeropixel shift technology. Nosepiece movement, manual, manual, automated motorized, manual. Block the fluorescence light by pulling out the block positioned on the upper right of the microscope. The leica dm500 fluorescence microscope upgrades the leica dm500 brightfield microscope to a single, dual, or three wavelength led fluorescence system.
The leica application suite las microscope software offers fully developed features for visualization, archiving, analysis and detailed documentation for routine tasks. For more information, please visit us at leica dmilm. This manual contains important instructions and information on the operating safety and maintenance of the. Roger robbins 5172006 table of contents leica inm100 optical microscope operation manual purpose introduction microscope description general microscope operation startup stage. Buy a microscope leica microscopes microscope central. Leica dm500 user manual safety regulations 9 instructions on use the leica dm500 microscope may be used only in closed rooms and must be placed on a solid substrate. However, there are two main features that sets fluorescent microscope apart from the traditional microscope. Upgrading a cheap microscope lets you see rainbows. Leica dmil inverted trinocular phase contrast integrated modulation contrast fluorescence microscope. The fluorescence illuminator for the leica dmlb laboratory microscope accommodates up to four filter cubes in horizontal positions in a rotating turret. Leica microsystems wetzlar gmbh ernstleitzstrasse d35578. Dm2500 ergonomic system microscope from leica biosystems offers you a. If the next user is coming within approximately 1hr and will be using fluorescence leave the microscope. The leica mseries filter changer can accommodate up to four filter combinations excitation and blocking filters.
These advantages make it possible to manipulate, sort and process specimens. It is easiest to choose tldic or fluo from the contrast method. Fluorescence microscopy solutions leica microsystems. Mz10 f fluorescence stereomicroscope and the leica fluorescence. It is used for examinations of cells and tissue, for micromanipulation and microinjection techniques all the way through to microdissection or confocal microscopy. It is easiest to choose tldic or fluo from the contrast method options in the software, press the eyepiece button and the shutter button to turn the light one. Straightforward setup and control of experiments is becoming more and more important in fluorescence microscopy. Leica dm2500 upright fluorescent microscope manuals cell. Las x navigator the latest addition to las x software is the las x navigator, a key to all applications on leica upright microscopes. Las x guides you from the setup to the analysis of the imaging data. Positions 25 are occupied by fluorescence filter cubes which cause a bias in the color of. Leica dm il fluorescence microscope available from the lab world group. View and download leica dmi3000b instructions manual online.
Log in your bnrf crl fl scopeplate reader session on the fom. This device and the filter cubes are identical to the corresponding components of leica microscopes for. Positions 25 are occupied by fluorescence filter cubes which cause a bias in the color of the images in transmission. Capture imagestimelapse movies with the camera x to capture images, ask assistance to have the leica. The leica dmi3000 b research microscope supports all transmitted light methods including fluorescence, live cell, timelapse imaging, highspeed multi fluorescence optical sectioning.
Leica dmi8 instructions for use manual pdf download. In such cases the declaration of conformity shall cease to be valid. Leica biosystems is proud to introduce its newest cytovision release, offering you the ability to bring your fish workflow out of the dark room and into the light. Upright microscopes leica biosystems offers a broad selection of clinical microscopes systems, from routine microscopes through to highly automated advanced solutions. Good to know that all microscopes cameras from leica biosystem scan be adapted to dm and dm2000 abroad selection of routine to highperformance brightfield and fluorescence. All microscope techniques and the required accessories for the leica dmil are described and explained in detail in the operation section of this manual, including the associated functions and their use.
Leica dm500 leica fluorescence microscope microscope central. All microscope techniques and the required accessories for the leica dmil are described and explained in detail in the operation section of this manual. User manual the present user manual describes only the functions of the fluorescence illuminator, the use of the filter sets, and the fitting of the fluorescence. Bright field, dic differential interference contrast, fluorescence hg lamp. This modulare product range can be customized to your needs with ergonomic accessories, special bases and extensive illumination options. Upright materials microscope with led illumination leica dm2700 m duration. This manual is an integral part of the leica dmirb microscope and must be read carefully before you start using the microscope. With exciting new features which improve image capture, expand on the dual reader workflow and provide you with images that mimic what you see at the microscope. Leica m165 fc leica m205 fa user manual microscopy and. One is the type of light source and the other is the use of specialized filter elements.
1085 131 1006 1278 381 230 1431 1188 1425 242 1357 1282 364 184 334 802 915 129 407 654 299 859 1297 206 1208 97 246